Safety assessment of different extracts of Linaria vulgaris Mill. in models of cytotoxicity and acute toxicity
DOI:
https://doi.org/10.31489/2026feb2/118-124Keywords:
Linaria vulgaris Mill., medicinal plant, extract, acute toxicity, cytotoxicityAbstract
The paper presents the results of a study on the acute toxicity and cytotoxic activity of extracts from the aboveground part of Linaria vulgaris Mill., collected during the flowering phase in the steppe areas of the Bukhar-Zhyrau district of the Karaganda region (Republic of Kazakhstan). The extracts were obtained by maceration, percolation, and ultrasonic extraction using various organic solvents (ethanol, ethyl acetate, and chloroform). Acute toxicity was studied in outbred white mice weighing 18–25 g after a single intragastric administration at doses of 500, 1500, and 2500 mg/kg. Cytotoxic activity was assessed using the Artemia salina larvae lethality assay. The data obtained showed that the 70 % ethanol extract of Linaria vulgaris Mill. belongs to the category of practically non-toxic substances and is classified as low-toxic (toxicity class III). The study of cytotoxic activity demonstrated that the Linaria vulgaris Mill. extracts, regardless of the method of preparation or the extractants used, do not exhibit toxic effects against Artemia salina larvae. Thus, the studied extracts of the aboveground part of Linaria vulgaris Mill. are characterized by low toxicity and lack of cytotoxic activity, which indicates their potential safety and prospects for further biological and pharmacological studies.
References
1 Cheriet T. Chemical constituents and biological activities of the genus Linaria(Scrophulariaceae) / T. Cheriet, I. Mancini, R. Seghiri, F. Benayache, S. Benayache // Natural Product Research. — 2015. — Vol. 29, No. 17. — P. 1589–1613. DOI: 10.1080/14786419.2014.999243. DOI: https://doi.org/10.1080/14786419.2014.999243
2 Флора Казахстана. — Т. 8. — Алма-Ата: Наука, 1965. — 34 с.
3 Akkol E. K. Antinociceptive and anti-inflammatory activities of some Linaria species from Turkey / E. Akkol, D. Ercil // Pharmaceutical Biology. — 2009. — Vol. 47, No. 3. — P. 188–194. DOI: https://doi.org/10.1080/13880200802465165
4 Utlu M. Phytochemical content, in vitro antioxidant and cholinesterase inhibitory activities of endemic Linariacorifolia Desf / M. Ultu, D. Ercil // Journal of Evidence-Based Complementary & Alternative Medicine. — 2024. DOI: 10.1177/1934578X241272734. DOI: https://doi.org/10.1177/1934578X241272734
5 Hua H.M. Study on the chemical constituents of Linaria vulgaris Mill. / H.M. Hua, X. Li, S.E. Xing, Y.H. Pei // Zhongguo Yaoxue Zazhi. — 2005. — Vol. 40, No. 9. — P. 653–656.
6 Hua H. A new pyrroloquinazoline alkaloid from Linaria vulgaris / H. Hua, M. Cheng, X. Li, Y. Pei // Chem Pharm Bull. — 2002. — Vol. 50, No. 10. — P. 1393-1394. DOI: https://doi.org/10.1248/cpb.50.1393
7 Ueyli A.K. Antimicrobial activity of secondary metabolites isolated from Linaria vulgaris Mill. / A.K. Ueyli, A.O. Ueyli, T.V. Bombela et al. // Khimiya rastitelnogo syrya. — 2024. — No. 4. — P. 287–296. DOI: https://doi.org/10.14258/jcprm.20240414795
8 Itzhanova Kh.I. Histochemical Analysis of Raw Materials of Linaria vulgaris Mill. growing in Central Kazakhstan / Kh.I. Itzhanova, D.K. Nurkadirov, M. Ishmuratova, A.B. Kosherber, S.A. Baidullayeva, E. Poleszak // Research Journal of Pharmacy and Technology. — 2024. — Vol. 17, No. 9. — P. 4477–4480. DOI: 10.52711/0974-360X.2024.00692. DOI: https://doi.org/10.52711/0974-360X.2024.00692
9 Итжанова Х. Анатомическое исследование растении льнянки обыкновенной (Linaria vulgaris Mill.), произрастающей в Центральном Казахстане / Х.И. Итжанова, Д.К. Нуркадыров, М.Ю. Ишмуратова, Г.М. Абдрахманова, Е.А. Тулебаев, А.Б. Кошербек // Фармация Казахстана. — 2024. — № 6. — С. 297–302. DOI: 10.53511/pharmkaz.2025.36.51.039. DOI: https://doi.org/10.53511/pharmkaz.2025.36.51.039
10 Pappachen L. K. Phytochemical screening and in vitro cytotoxicity studies of Mussaendafrondosa Linn leaves / L.K. Pappachen, K.S. Sreelakshmi // Research Journal of Pharmacy and Technology. — 2017. — Vol. 10, No. 12. — P. 4227–4230. DOI: 10.5958/0974-360X.2017.00774.0. DOI: https://doi.org/10.5958/0974-360X.2017.00774.0
11 Johnson D. B. Antidiabetic activity and toxicity studies of ethanolic extract of Polyalthialongifolia roots / D.B. Johnson, A. Raj, R. Venkatanarayan // Research Journal of Pharmacy and Technology. — 2017. — Vol. 10, No 5. — P. 1305–1312. DOI: https://doi.org/10.5958/0974-360X.2017.00231.1
12 Миронов А.Н. Руководство по проведению доклинических исследований лекарственных средств / А.Н. Миронов. — М.: Гриф и К, 2012. — Ч. 1. — С. 13–41.
13 Руководство по экспериментальному (доклиническому) изучению новых фармакологических веществ / под общ. ред. Р.У. Хабриева. — 2-е изд., перераб. и доп. — М.: ОАО «Издательство “Медицина”», 2005. — 832 с.
14 Nurkadirov D.K. Quantitative determination of flavonoids from the aerial parts of Linaria vulgaris Mill. / D.K. Nurkadirov, Kh.I. Itzhanova // International Scientific and Technical Conference “Actual Problems of the Chemistry of Natural Compounds”. — Tashkent, 2024. — P. 179.
15 Nurkadirov D.K. Study of amino acid composition of Linaria vulgaris Mill. / D.K. Nurkadirov, Kh.I. Itzhanova, A. Kosherbek // International Scientific and Practical Online Conference “Modern Achievements of Experimental, Clinical, Environmental Biochemistry and Molecular Biology”. — Ukraine, 2024. — P. 408–410.
16 Nurkadirov D.K. Analysis of the chemical composition of the essential oil of Linaria vulgaris Mill. / D.K. Nurkadirov, Kh.I. Itzhanova // Silk Road Chemistry: Modern Chemical Technologies and Science: Collection of abstracts of the 1st International Scientific and Practical Conference, dedicated to the 100th anniversary of Academician Ye.A. Buketov. — Karaganda. — 2025. — P. 164-165.
