Morphological and biochemical analysis of hawthorn and rosehip of the Ulytau region
DOI:
https://doi.org/10.31489/2025feb4/65-75Keywords:
Ulytau region, Crataegus chlorocarpa, Rosa species, morphology, biochemistry, vitamin C, anthocyanins, cluster analysisAbstract
The study investigates the morphological and biochemical profiles of two forms of Crataegus chlorocarpa and five forms of Rosa species (R. laxa, R. acicularis, R. spinosissima) from natural populations in the Zhanaarka district, Ulytau region, Kazakhstan. Geographical coordinates, shrub height, age, crown shape, yield, fruit size, color, and taste were assessed. Biochemical analyses quantified vitamin C, anthocyanins, leucoanthocyanidins, catechins, sugars, pectins, and titratable acidity using standardized methods. Rosa acicularis-058 showed the highest vitamin C content (576.4 mg/100 g FW), while Rosa spinosissima-064 ex[1]celled in anthocyanins (1259.3 mg/100 g FW). Crataegus forms were notable for their catechin content (up to 242.3 mg/100 g FW). Pearson’s correlation revealed strong positive correlations between vitamin C and leucoanthocyanidins (r=0.83, p=0.02), and between titratable acidity and sugars (r=0.86, p=0.01).UPGMA cluster analysis identified three distinct groups based on biochemical traits. These findings highlight the potential of these wild plants for nutraceutical development and breeding, as they surpass some conventional crops in bioactive compounds.
References
1 Maksutbekova, G.T., & Akhmatov, M.K. (2017). Dikorastushchie drevesno-kustarnikovye rasteniia Zhezkazganskogo regiona i ikh primenenie v ozelenenii [Wild woody and shrubby plants of the Zhezkazgan region and their using in green building]. Mezhdunarodnyi nauchno-issledovatelskii zhurnal — International scientific and research journal, 11 (65), 25‒32. https://doi.org/10.23670/IRJ.2017.65.146 [in Russian].
2 Dzhangaliev, A.D., Salova, T.N., & Turechanova, R.M. (2001). Dikie plodovye rasteniia Kazakhstana [Wild fruit plants of Kazakhstan]. Almaty [in Russian].
3 Imanbayeva, A., Duisenova, N., Orazov, A., Sagyndykova, M., Belozerov, I., & Tuyakova, A. (2024). Study of the Floristic, Morphological, and Genetic (atpF–atpH, Internal Transcribed Spacer (ITS), mat K, psbK-psbI, rbcL, and trnH-psbA) Differences in Crataegus ambigua Populations in Mangistau (Kazakhstan). Plants, 13, 1591. https://doi.org/10.3390/plants13121591. DOI: https://doi.org/10.3390/plants13121591
4 Ishmuratova, M.Yu., Myrzaly, G.Zh., Iblev, V.I., & Matveev, A.N. (2016). Flora gor Ulytau (Tsentralnyi Kazakhstan) [Flora of the Ulytau Mountains (Central Kazakhstan)]. Karaganda: RIO Bolashak-Baspa [in Russian].
5 Jiménez-López, J., Ruiz-Medina, A., Ortega-Barrales, P., & Llorent-Martínez, E.J. (2017). Rosa rubiginosa and Fraxinus oxycarpa herbal teas: characterization of phytochemical profiles by liquid chromatography-mass spectrometry, and evaluation of the antioxidantactivity. New J. Chem., 41, 7681–7688. DOI: https://doi.org/10.1039/C7NJ01743J
6 Smanalieva, J., Iskakova, J., Oskonbaeva, Z., Wichern, F., & Darr, D. (2020). Investigation of nutritional characteristics and free radicalscavenging activity of wild apple, pear, rosehip, and barberry from the walnut-fruit forests of Kyrgyzstan. Eur. Food Res. Technol., 246, 1095–1104. DOI: https://doi.org/10.1007/s00217-020-03476-1
7 Peña, F., Valencia, S., Tereucán, G., Nahuelcura, J., Jiménez-Aspee, F., Cornejo, P., & Ruiz, A. (2023). Bioactive Com pounds and Antioxidant Activity in the Fruit of Rosehip (Rosa canina L. and Rosa rubiginosa L.). Molecules, 28, 3544. https://doi.org/10.3390/molecules28083544. DOI: https://doi.org/10.3390/molecules28083544
8 Sagbas, H.I. (2023). Investigation of Fruit Quality and Biochemical Traitsof Rosehip (R. canina) Ecotypes in the Aegean Region of Türkiye. Horticulturae, 9, 1292. https://doi.org/10.3390/horticulturae9121292. DOI: https://doi.org/10.3390/horticulturae9121292
9 Tomar, O. (2023). Determination of some quality properties and antimicrobial activities of kombucha tea prepared with different berries. Turk. J. Agric. For, 47, 252–262. DOI: https://doi.org/10.55730/1300-011X.3083
10 Murathan, Z.T., Zarifikhosroshahi, M., & Kafkas, N.E. (2016). Determination of fatty acids and volatile compounds in fruits of rosehip (Rosa L.) species by HS-SPME/GC-MS and Im-SPME/GC-MS techniques. Turk. J. Agric. For., 40, 269‒279. DOI: https://doi.org/10.3906/tar-1506-50
11 Grudzinskaya, L.M., Gemedzhieva, N.G., Nelina, N.V., & Karzhaubekova, Zh.Zh. (2014). Annotirovannyi spisok lekarsvennykh rastenii Kazakhstana [Annotated list of medicinal plants of Kazakhstan]. Almaty [in Russian].
12 Balta, M.F., Çelik, F., Türkoğlu, N., Özrenk, K., & Özgokçe, F. (2006). Some fruit traits of hawthorn (Crataegus spp.) genetic resources from Malatya, Turkey. Res. J. Agric. Biol. Sci., 2, 531‒536.
13 Guo, T.J. (1995). Hawthorn (Crataegus) Resources in China. Hort Sci., 30, 1132‒1134. DOI: https://doi.org/10.21273/HORTSCI.30.6.1132
14 Khadivi, A., Heidari, P., Rezaei, M., Safari-Khuzani, A., & Sahebi, M. (2019). Morphological variabilities of Crataegus monogyna and C. pentagyna in northeastern areas of Iran. Industrial Crops and Products, 139, 111531. https://doi.org/10.1016/j.indcrop.2019.111531. DOI: https://doi.org/10.1016/j.indcrop.2019.111531
15 Prior, R.L., Cao, G., Martin, A., Sofic, E., McEwen J., O’Brien, C. et al. (1998). Antioxidant capacity is influenced by total phenolic andanthocyanin content, maturity, and variety of Vaccinium species. J Agric Food Chem, 46, 2686‒2693. DOI: https://doi.org/10.1021/jf980145d
16 Cevallos-Casals, B.A., Byrne, D., Okie, W.R., & Cisneros-Zevallos, L. (2006). Selecting new peach and plum genotypes rich in phenoliccompounds and enhanced functional properties. Food Chem., 96, 273‒280. DOI: https://doi.org/10.1016/j.foodchem.2005.02.032
17 Ercişli, S, Orhan, E, Özdemir, O., & Şengül M. (2007). The genotypic effects on the chemical composition and antioxidant activity of sea buckthorn (Hippophae rhamnoides L.) berries grown in Turkey. Sci. Hortic., 15, 27‒33. DOI: https://doi.org/10.1016/j.scienta.2007.07.004
18 Mukan, G., Shadmanova, L., Sankaybayeva, A., Ugit, L., Razhanov, M., & Yeszhanova, A. (2025). Survey, selection, and study of promising forms of sea buckthorn (Hippophae rhamnoides L.) in Burabay National Park. Pak. J. Bot., 57(6). http://dx.doi.org/10.30848/PJB2025-6(13). DOI: https://doi.org/10.30848/PJB2025-6(13)
19 Beydeman, I.N. (2004). Metodika izucheniia fenologii rastenii i rastitelnykh soobschestv [Methodology for studying the phenology of plants and plant communities]. Novosibirsk. Nauka [in Russian].
20 Sapozhnikova, E.V., & Dorofeeva, L.S. (1966). Opredelenie soderzhaniia askorbinovoi kisloty v okrashennykh rastitelnykh ekstraktakh iodometricheskim metodom [Determination of ascorbic acid content in coloured plant extracts using the iodometric method]. Konservatsiia i ovoshchesushilnaia promyshlennost — Preservating and vegetable drying industry, 5, 29–31 [in Russian].
21 Hagerman, A. (2002). Tannin Chemistry. Retrieved from http://www.users.muohio.edu/hagermae/tannin.
22 Skorikova, Yu.G., & Shaftan, E.A. (1968). Metodika opredeleniia antotsiantov v plodakh i yagodakh [Methodology for de termining anthocyanins in fruits and berries]. Trudv III Vserossiiskogo seminara po biologicheski aktivnim (lechebnym) veshchestvam plodov i yagod — The collection of works of the Third All-Union Seminar on Biologically Active (Medicinal) Sub stances in Fruits and Berries, 451. Sverdlovsk, 451 [in Russian].
23 Hagerman, A., Harvey-Mueller, I., & Makkar, H.P.S. (2000). Quantification of tannins in tree foliage laboratory manual. FAO/IAEA. Vienna.
24 Ermakov, A.I. (1987). Metody biokhimicheskogo analiza rastenii [Methodology for biochemical analysis of plants]. Leningrad [in Russian].
25 Zhang, L.F., Wang, P., Chen, F.S., Lai, S.J., Yu, H.G., & Yang, H.S. (2019). Effects of calcium and pectin methylesterase on quality attributes and pectin morphology of jujube fruit under vacuum impregnation during storage. Food Chemistry, 289, 40‒48. https://doi.org/10.1016/j.foodchem.2019.03.008. DOI: https://doi.org/10.1016/j.foodchem.2019.03.008
26 Pavilionov, A.A., & Rozhkov, I. (1986). Novye plodovye yagodnye kultury [New fruit and berry crops]. Moscow: Rosselkhozizdat [in Russian].
27 Bakhtiar, Z., Eghlima, G., Hatami, M., & Mirjalili, M.H. (2023). Quantification of fatty acids in seed oil and important bioactive compounds in Iranian Rosa canina L. ecotypes for potential cosmetic and medicinal uses. Sci. Rep., 13(1); 22721. https://doi.org/10.1038/s41598-023-50135-y. DOI: https://doi.org/10.1038/s41598-023-50135-y
28 Roman, I, Stănilă, A., & Stănilă, S. (2013). Bioactive compounds and antioxidant activity of Rosa canina L. biotypes from spontaneous flora of Transylvania. Chem. Cent. J., 7(1), 73. https://doi.org/10.1186/1752-153X-7-73. DOI: https://doi.org/10.1186/1752-153X-7-73
29 Sevindik, E. (2016). Characterization of bioactive compounds in rosehip species from the East Anatolia region of Turkey. Italian Journal of Food Science, 28(2), 314‒325. https://doi.org/10.14674/1120-1770/ijfs.v198.


